Content and distribution of inorganic elements in Laminaria japonica based on ICP-MS and Micro-XRF / 中国中药杂志

In order to evaluate the composition and distribution characteristics of inorganic elements in Laminaria japonica, this study employed inductively coupled plasma mass spectrometry(ICP-MS) to detect the inorganic elements and used high performance liquid chromatography tandem ICP-MS(HPLC-ICP-MS) to determine the content of different arsenic species in L. japonica from diffe-rent origins. Micro X-ray fluorescence(Micro-XRF) was used to determine micro-area distribution of inorganic elements in L. japonica. The results showed that the average content of Mn, Fe, Sr, and Al was high, and that of As and Cr exceeded the limits of the national food safety standard. According to the results of HPLC-ICP-MS, arsenobetaine(AsB) was the main species of As contained in L. japonica. The more toxic inorganic arsenic accounts for a small proportion, whereas its content was 1-4 times of the limit in the national food safety standard. The results of Micro-XRF showed that As, Pb, Fe, Cu, Mn, and Ni were mainly distributed on the surface of L. japonica. Among them, As and Pb had a clear tendency to diffuse from the surface to the inside. The results of the study can provide a basis for the processing as well as the medicinal and edible safety evaluation of L. japonica.

Development of Fucosterol Certified Reference Material / 中国实验方剂学杂志

Objective:According to the GB/T 15000.3-2008， to develop a fucosterol certified reference material based on the project approved by Standardization Administration. Method:Fucosterol was isolated from <italic>Laminaria japonica</italic> dried thallus via 95% ethanol extraction， vacuum concentration， repeated column chromatography separation， recrystallization in petroleum ether-ethyl acetate， and residual solvent removal. Its chemical structure was identified by elemental analysis （EA）， infrared spectrum （IR）， mass spectrometry （MS）， nuclear magnetic resonance （NMR） and X-ray diffraction （XRD）. Its homogeneity， stability， and cooperative certification conducted by 8 laboratories were carried out by high performance liquid chromatography with evaporative light scattering detector. Result:For the fucosterol reference material， the certified value of purity was 99.54% with expanded uncertainty of 0.16% in confidence interval of 95%， the stability was good within 24 months storage period at 2-4 ℃， which met the technical requirements of reference material and passed the acceptance organized by Standardization Administration. Conclusion:The national standard materials of fucosterol has been successfully developed， which can be used for the determination of this component， the evaluation of detection methods， and the detection and quality control of related products.

Secondary metabolites of endophyte Alternaria sp. W-1 from Laminaria japonica / 药学学报

Six compounds were isolated from the crude extract of the liquid culture of Alternaria sp. W-1 by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC. They were identified as 6-iso-tricycloalternarene 6a (1), tricycloalternarene 6a (2), tricycloalternarene B (3), uracil (4), 5-methyluracil (5), and lumichrome (6) through HR-MS, NMR and literature comparison. 6-iso-Tricycloalternarene 6a (1) is a new compound which has never been reported in the literature. In cytotoxicity assay, compounds 1-3 showed weak inhibition activity to human hepatoma cell line SMMC-7721 and human gastric cell line SGC-7901.

Effect of laminaria japonica polysaccharides on laryngeal carcinoma cell proliferation and apoptosis in vitro / 中国耳鼻咽喉头颈外科

OBJECTIVE To investigate the effect and its mechanism of laminaria japonica polysaccharides(LJP) on human laryngeal carcinoma cell Tu212 growth. METHODS The laryngeal squamous cell Tu212 in logarithmic growth phase were treated by laminaria japonica polysaccharides(80, 160, 320 μg/ml) and cisplatin(1.8 mg/L). The MTT assay was used to evaluate cell growth inhibition rate after 24h, 48h and 72h. The flow cytometry with PI staining were used to estimate cell cycle distribution after 48h. The fluorescent microscopy was used to estimate cell apoptosis after 48h. The western blot was used to evaluate the expressions of Cyclin B1 and Bcl-2. RESULTS The cell proliferation was inhibited by cellular LJP in a dose and time dependent manner. Tu212 cells proliferation were stopped at the G2/M phase treated with LJP after 48h. Cell apoptosis was observed clearly by f luorescent microscope after 48h. The expressions of Cyclin B1 and Bcl-2 were suppressed significantly treated with LJP after 48 h. All of the differences were significant(P <0.05). CONCLUSION LJP has inhibitive effects on the Tu212 cells, this mechanism was probably through inhibition of the proliferation and promotion of the apoptosis.

Influence on compatibility of Glycyrrhiza uralensis and Laminaria japonica on liver and kidney functions and serum indexes in rats / 中草药

Objective To evaluate the effects of compatibility of Glycyrrhiza uralensis and Laminaria japonica on liver and kidney functions as well as serum indexes in rats. Methods A total of 24 male SD rats were randomly divided into four groups. Group 1 was served as control and only received vehicle. Group 2 and group 3 were orally dosed of G. uralensis extracts (2.8 g/kg) and L. japonica extracts (3.8 g/kg) once daily, respectively. Group 4 was orally dosed with 6.8 g/kg of G. uralensis-L. japonica extracts once daily. The experimental rats were treated corresponding extracts or vehicle for 17 weeks. During the experiment, the weight of rats, organ coefficient, biochemical indexes, and liver histopathological photograms in each group were measured. Meanwhile, plasma glycyrrhetinic acid concentration in G. uralensis extract group and G. uralensis-L. japonica extract group were observed. Results The water extraction components from G. uralensis and L. japonica groups could significantly reduce the level of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), cholinesterase (CHE), total bile acid (TBA), and total bilirubin (TBIL) comparing with control group (P < 0.05). While the G. uralensis-L. japonica extracts group could reverse these biochemistry indexes. G. uralensis-L. japonica extracts markedly increased the plasma concentration and exposure of glycyrrhetinic acid. Electrolyte metabolism balance was disordered after long-term treatment of G. uralensis, L. japonica and G. uralensis-L. japonica extracts, showing the level of sodium (Na+), potassium (K+), and chloride ion (Cl-) in these three groups were significantly higher than that in control group. Conclusion These results indicated that G. uralensis or L. japonica extracts might has hepatoprotective effects. However, G. uralensis-L. japonica extracts attenuated the hepatoprotective effects, which might result from the increased plasma concentration of glycyrrhetinic acid.

Laminaria japonica increases plasma exposure of glycyrrhetinic acid following oral administration of Liquorice extract in rats / 中国天然药物

The present study was designed to investigate the effects of Laminaria japonica (Laminaria) on pharmacokinetics of glycyrrhetinic acid (GA) following oral administration of Liquorice extract in rats. Following oral administrations of single-dose and multi-dose Liquorice extract and Liquorice-Laminaria extract, respectively, plasma samples were obtained at various times and the concentrations of GA, liquiritigenin, and isoliquiritigenin were measured by LC-MS. The effects of Laminaria extract on pharmacokinetics of GA were also investigated, following single-dose and multidose of glycyrrhizic acid (GL). The effects of Laminaria extract on intestinal absorption of GA and GL were studied using the in situ single-pass intestinal perfusion model. The metabolism of GL to GA in the contents of small and large intestines was also studied. The results showed Liquorice-Laminaria extract markedly increased the plasma concentration of GA, accompanied by a shorter Tmax. Similar alteration was observed following multidose administration. However, pharmacokinetics of neither liquiritigenin nor isoliquiritigenin was affected by Laminaria. Similarly, Laminaria markedly increased concentration and decreased Tmax of GA following oral GL were observed. The data from the intestinal perfusion model showed that Laminaria markedly increased GL absorption in duodenum and jejunum, but did not affect the intestinal absorption of GA. It was found that Laminaria enhanced the metabolism of GL to GA in large intestine. In conclusion, Laminaria increased plasma exposures of GA following oral administration of liquorice or GL, which partly resulted from increased intestinal absorption of GL and metabolism of GL to GA in large intestine.

Effectiveness of the combinational treatment of Laminaria japonica and Cistanche tubulosa extracts in hair growth / 한국실험동물학회지

Since scalp hair loss has increased recently even in young people, seriously affecting individual's quality of life, the hair growth-stimulating effects of Laminaria japonica extract (LJE) and Cistanche tubulosa extract (CTE) were investigated. After confirming anagen phase of follicles under shaving, male C57BL/6 mice were dermally applied with 3% Minoxidil or orally administered with the combinations of LJE and CTE for 21 days. Minoxidil promoted the hair regrowth and increased gamma-glutamyl transpeptidase (gamma-GTP) and alkaline phosphatase (ALP) activities. In addition, Minoxidil up-regulated epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) levels. Co-administration of LJE and CTE at 54 mg/kg LJE plus 162 mg/kg CTE exerted synergistic promoting effects on the hair regrowth, comparable to 3% Minoxidil. LJE preferentially enhanced ALP activity, while CTE increased both gamma-GTP and ALP activities as well as EGF and VEGF expressions. In vivo air pouch inflammation model, carrageenan-induced vascular exudation and increased nitric oxide and prostaglandin E2 concentrations in the exudates were synergistically suppressed by co-administration of LJE and CTE. In addition, inflammatory cell infiltration was substantially inhibited by the combinational treatment. The results suggest that combinational oral treatment with LJE and CTE in appropriate doses and ratios prevent hair loss and improve alopecia, which might be in part mediated by their anti-inflammatory activities.

Comparative analysis of anti-Helicobacter pylori activities of FEMY-R7 composed of Laminaria japonica and Oenothera biennis extracts in mice and humans / 한국실험동물학회지

Helicobacter pylori-eliminating effects of FEMY-R7, composed of Laminaria japonica and Oenothera biennis extracts, were investigated in mice and humans. Male C57BL/6 mice were infected with the bacteria by intragastric inoculation (1x10(9) CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with total 20, 64 or 200 mg/kg/day FEMY-R7 for 2 weeks. In Campylobcter-like organism (CLO)-detection tests on gastric mucosa and feces, FEMY-R7 reduced the urease-positive reactivity in a dose-dependent manner; i.e., the positivity ratios were decreased to 70, 20, and 10% for gastric mocosa and to 80, 50, and 20% for feces. In a clinical sudy, human subjects, confirmed to be infected with Helicobacter pylori, were orally administered twice a day with capsules containing total 100, 320 or 1,000 mg/man/day FEMY-R7 (matching doses for 20, 64 or 200 mg/kg/day, respectively, in mice from a body surface area-based dose translation) for 8 weeks. FEMY-R7 decreased the positivity ratios in feces to 70, 40, and 30%, respectively. In bacterial culture, H. pylori was identified from the CLO-positive stools of mice and humans. The bacterial identification ratios exhibited a good correlation between the matching doses in mice and humans. It is suggested that FEMY-R7 could be a promising functional food without tolerance as an adjunct to reduce the dosage of antibiotics for the treatment of recurrent H. pylori infection.

Efficacy of Cistanche Tubulosa and Laminaria Japonica Extracts (MK-R7) Supplement in Preventing Patterned Hair Loss and Promoting Scalp Health

Cistanche tubulosa and Laminaria japonica have been reported to have anti-oxidative, anticoagulant, anti-cancer and anti-inflammatory properties. They are expected to be a promising candidates for promoting hair growth and treating dandruff and scalp inflammation as a consequence. In this double-blinded, placebo-controlled clinical trial, we investigated the efficacy of Cistanche tubulosa extract and Laminaria japonica extract complex (MK-R7) in promoting hair health in patients with mild to moderate patterned hair loss. Using phototrichogram (Folliscope 4.0, LeadM, Seoul, Korea), we compared the density and diameter of hairs in patients receiving a placebo or Cistanche tubulosa extract and Laminaria japonica extract complex (MK-R7) at baseline, 8 and 16 weeks of the study. In order to determine the efficacy of treatment on dandruff and scalp inflammation, investigator's assessment score and patient's subjective score were also performed. We found a statistically significant increase in the hair density of the test group (n = 45, MK-R7 400 mg) after 16 weeks of consuming the MK-R7 (test group: 23.29 n/cm2 +/- 24.26, control: 10.35 n/cm2 +/- 20.08, p < 0.05). In addition, we found a statistically significant increase in hair diameter in the test group compared to control group at week 16 (test group: 0.018 mm +/- 0.015, control: 0.003 mm +/- 0.013, p < 0.05). There were also significant outcomes regarding the investigator's visual assessment and patient's subjective score of dandruff and scalp inflammation in the test group compared to those in control group. Based on the results of this clinical study, we conclude that Cistanche tubulosa extract and Laminaria japonica extract complex (MK-R7) are promising substances for promoting health of the scalp and hair.

The hypoglycemic effect and mechanism of Laminaria japonica on diabetic model induced by alloxan in rats / 国际中医中药杂志

Objective To investigate the hypoglycemic effects of Laminaria japonica (L. japonica) on diabetic model induced by alloxan in rats. Methods Sixty healthy female rats were used to establish diabetic models by injecting alloxan peritoneally, and L.japonica was applied as raw materials for potential marine drugs.The levels of fasting blood glucose (FBG) were detected by automatic blood glucose device. Enzyme linkedimmunoabsorbant assay was applied to determine the insulin level in serum. The shape and structure of isletcells were observed with histopathological staining, and the expression of superoxide dismutase (SOD) and inducible nitric oxide synthase (iNOS) in islet cells were detected by immunohistochemical technique. Results After the treatment, the levels of FBG of L.japonica treated group B [(9.37±1.70) mmol/LandC (9.18±1.65 ) mmol/L, F= 32.81, q=6.35～11.72, P<0.05 ] reduced, while the serum levels of insulin in treated group A, Band C (0.0378±0.0026, 0.0378±0.0027, 0.0367±0.0035) increased(F= 11.40, q=4.28～8.47, P<0.05) significantly than those of diabetic model group (0.0456 ±0.0057) . The shape and structure of islet cells improved with the up-expressing SOD(t=4.73～4.76, P<0.05)and down-expressing iNOS (t=4.81～5.30, P<0.05) in L.japonica treated group B and C than those in diabetic model group. Conclusion L.japonica might decrease the serum level of FBG through promoting the islet cell recovery by an anti-oxide effect.

Effect of Glutathione With Sea Tangle Extract on Prevention of Selenite-Induced Cataract Formation in Rats

PURPOSE: To evaluate antioxidative and preventive effects of sea tangle extract on selenite-induced cataract formation. METHODS: Eighty SD rat pups were randomized into 8 groups. Group 1 received no injection of reagent (normal); Group 2 to 8 received injection of selenite (15 micromol/Kg, s.c.) was injected. In group 2 (control) and group 3, normal saline (i.p.) and ascorbic acid (i.p.) was injected on days 3~31. In groups 4~8, sea tangle extract (i.p.) was injected at a concentration of 12.5, 25, 50, 100, 200 mg/kg, respectively. Development of cataract was assessed and photographed weekly under slit lamp. Rat lenses were analyzed for antioxidant enzymes, glutathione peroxidase (GPx), superoxide dismutase and malondialdehyde. Furthermore, an amino acid analysis of sea tangle extract was performed. RESULTS: Significant differences (p<0.05) were seen in cataract development in group 7. Dense nuclear cataracts developed in 8 of 10 of the control group (group 2); Group 4~8 developed nuclear cataract with proportion of 6/10, 3/10, 2/10, 1/10, and 6/10 rats. In sea tangle injected group, levels of GPx were higher than in the ascorbic acid and control groups. In particular, group 7, injected with 100 mg/kg of sea tangle extract, showed significantly high level of enzyme. Results of the amino acid analysis showed sea tangle includes glutamate-glycine-cysteine, major constituents of glutathione (GSH). CONCLUSIONS: The glutamate-glycine-cysteine in sea tangle is supposed to increase the level of lens GSH and this may contribute to lowering cataract development. This study strongly supports the activity of sea tangle as an endogenous antioxidant and anticataract agent.

Photolithotrophic Cultivation of Laminaria japonica Gametophyte Cells in Stirred Tank Photobioreactors: Studies in Different Pulse Feeding Modes / 中国生物工程杂志

Photoautotrophic gametophyte cells of the brown macroalgae Laminaria japonica were cultivated in 500ml stirred tank photobioreactors under seven pulse feeding modes and one batch mode.It is the first time for the study of effects of the feeding time points and feeding quantity on macroalgal cell growth and nutrient consumption.Results showed that, with inoculum density of 50mg DCW/L, in modified APSW artificial seawater medium at 13℃, light intensity of 60μE/m2.s, light cycle of 16/8h L/D, aeration rate of 50ml/min, and agitation speed of 100r/min, feeding the culture with small nutrient quantity was beneficial for the synchronization between nitrate and phosphate absorption, and further for biomass production.Feeding when ambient nutrient was abundant or depleted was quite weak for large amount of biomass accumulation, which might be due to the slowing nutrient absorption, nutrient storage, or the divergence absorption between nitrate and phosphate.Feeding nutrient frequently with small quantity from mid-exponential growth of macroalgal cells, that is maintaining medium nutrient concentration between 1/3 and 1/2 of its initial concentration, was the most effective way for biomass production, with biomass increased by 12.270 times of for 51 days' cultivation.

Photolithotrophic Cultivation of Laminaria japonica Gametophyte Cells in Stirred Tank Photobioreactors: Studies in Different Pulse Feeding Modes / 中国生物工程杂志

Photoautotrophic gametophyte cells of the brown macroalgae Laminaria japonica were cultivated in 500ml stirred tank photobioreactors under seven pulse feeding modes and one batch mode.It is the first time for the study of effects of the feeding time points and feeding quantity on macroalgal cell growth and nutrient consumption.Results showed that, with inoculum density of 50mg DCW/L, in modified APSW artificial seawater medium at 13℃, light intensity of 60μE/m2.s, light cycle of 16/8h L/D, aeration rate of 50ml/min, and agitation speed of 100r/min, feeding the culture with small nutrient quantity was beneficial for the synchronization between nitrate and phosphate absorption, and further for biomass production.Feeding when ambient nutrient was abundant or depleted was quite weak for large amount of biomass accumulation, which might be due to the slowing nutrient absorption, nutrient storage, or the divergence absorption between nitrate and phosphate.Feeding nutrient frequently with small quantity from mid-exponential growth of macroalgal cells, that is maintaining medium nutrient concentration between 1/3 and 1/2 of its initial concentration, was the most effective way for biomass production, with biomass increased by 12.270 times of for 51 days' cultivation.

Photolithotrophic Cultivation of Laminaria japonica Gametophyte Cells in Stirred Tank Photobioreactors: Studies in Different Pulse Feeding Modes / 中国生物工程杂志

Photoautotrophic gametophyte cells of the brown macroalgae Laminaria japonica were cultivated in 500ml stirred tank photobioreactors under seven pulse feeding modes and one batch mode.It is the first time for the study of effects of the feeding time points and feeding quantity on macroalgal cell growth and nutrient consumption.Results showed that, with inoculum density of 50mg DCW/L, in modified APSW artificial seawater medium at 13℃, light intensity of 60μE/m2.s, light cycle of 16/8h L/D, aeration rate of 50ml/min, and agitation speed of 100r/min, feeding the culture with small nutrient quantity was beneficial for the synchronization between nitrate and phosphate absorption, and further for biomass production.Feeding when ambient nutrient was abundant or depleted was quite weak for large amount of biomass accumulation, which might be due to the slowing nutrient absorption, nutrient storage, or the divergence absorption between nitrate and phosphate.Feeding nutrient frequently with small quantity from mid-exponential growth of macroalgal cells, that is maintaining medium nutrient concentration between 1/3 and 1/2 of its initial concentration, was the most effective way for biomass production, with biomass increased by 12.270 times of for 51 days' cultivation.

Optimization of Alginic Acid in Laminaria japonica Aresch by Orthogonal Test / 中国中医药信息杂志

Objective To optimize the extraction technology of alginic acid in Laminaria japonica Aresch. Methods The influence of the amount of solvent, the extraction time and temperature (3 factors) on the alginic acid content and yield was investigated by orthogonal test L9(34), and carried on to inspect the methodology. Result The optimum extraction condition was A1B1C2, that was adding 30 times solvent (1%Na2CO3), whisk extracting for one hour at 60 ℃. Conclusion The temperature is the most important factor. The content of alginic acid decrease obviously at above 60 ℃. The optimum extraction technology is reasonable.

RELATION OF ANTIRADIATION EFFECT OF LAMINARIA JAPONICA POLYSACCHARIDES AND LYMPHOCYTE APOPTOSIS / 营养学报

Objective:To investigate the relation of antiradiation effect of LJP and lymphocyte apoptosis. Methods:36 Wistar rats were randomly divided into 6 groups (n=6): control, model,and LJP given i.g.at 4 doses(100,200,300,400 mg/ kg bw) for 10d before whole-body irradiation with 9.0 Gy Co?-ray. 18h later,the effects of LJP on the indices of immune function of the irradiated rats 60 were measured. TUNEL and flow cytometry were used to study the effects of LJP on splenic lymphocyte apoptosis and immunohistochemical technique was used to detect the expression of Bcl-2 and the Bax protein. Results:LJP significantly modulated immune function in irradiated rats. The apoptosis ratio of splenic lymphocyte of the model group was higher than those of other groups. LJP could markedly inhibit the effects of irradiation on apoptosis and increase the ratio of bcl-2/bax protein in dose-effect manner. Conclusion:LJP could inhibit splenic lymphocyte apoptosis induced by irradiation, and its mechanism is associated with regulating the expression of bcl-2 and bax protein of splenic lymphocyte. Key word:laminaria japonica polysaccharides; irradiation; lymphocyte; apoptosis apoptosis-related genes

Protective effect of fucoidan from Laminaria japonica on acute liver injury in mice / 中国海洋药物

Objective To study the protective effects of fucoidan from Laminaria japonica on CCl_4-induced acute liver injury in mice.Methods Fifty mice were allocated into control group,model group,bifendate group,low and high dosage of fucoidan group randomly.The mice were treat with bifendate(100mg?kg~(-1)) or fucoidan(200,100mg?kg~(-1)) for 10 days. Then 10%CCl_4(10mL?kg~(-1)) was given to mice by intraperitoneal injection.The activities of serum ALT,AST,ALP and BIL were determined,the body weight and liver weight were measured,as well as the pathological changes of the liver were observed in all groups.Results fucoidan(200mg?kg~(-1)) could significantly lower the ALT,AST BIL in serum and the liver index of mice,improve the liver histology.Conclution fucoidan can protect the liver against damage induced by CCl_4 in mice.

Regulating effects and mechanism of Laminaria japonica on serum lipid of hyperlipidemia rats / 中国海洋药物

Objective To investigate the regulating effects and mechanism of Laminaria japonica (L.japonica) on serum lipid of hyperlipidemia rats.Methods Forty healthy female Wistar rats were used to establish hyperlipidemia models by feeding fat-rich forage,and the powder of L.japonica was applied as a supplement in forage for test groups.The levels of serum lipid including the triglyceride(TG),total cholesterol(TC),low-density lipoprotein (LDL),high-density lipoprotein(HDL) and the activities of lipoproteinesterase(LPL) and hepatic lipase(HL) were detected by biochemical assay.Results The levels of serum TG and TC in test group decreased significantly than those in pre-treated and model group (P

Preparation and in vitro antioxidative activity of acetylated fucoidan extracted from Laminaria japonica / 中国海洋药物

Objective To explore the optimum method for preparation of acetylated fucoidan extracted from Laminaria japonica,and test the antioxidative activity of acetylated fucoidans prepared under different conditions in vitro.Methods Using acetic anhydride as acetylated reagent,N-Bromosuccinimide(NBS) as catalyst,the effects of reaction time,temperature and volume of acetylated reagent were tested by the orthogonal design method.The antioxidative activity of the prepared acetylated fucoidans in vitro was determined,including scavenging activity against superoxide,hydroxyl and DPPH radical and reducing power.Results Volume of acetylated reagent and reaction temperature were the significant factors(P

Studies on the extraction of polysaccharides sulfate from three algae and their scavenging activity on free radicals / 中国海洋药物

Objective The polysaccharides sulfate were extracted from three kinds of algae in-cluding porphyra tenera , Laminaria ja ponica and Sargassum fusi forme ( Harv. )Setch . in order to screen antioxidant with exploiting potential. Methods Using the assay system of DP-PH,the antioxidative activities of various extracts were studied. Results Three kinds of algae polysaccharides sulfate had different antioxidative activities. Moreover, polysaccharide extracted with different solvents had different intensities of antioxidative activity. Conclusion Polysacchride sulfate extracted with water has stronger antioxidative activity than that with others.keywolds antioxidation; porphyra tenera kjellm. ; Laminaria japonica; Sargassum fusi -forme ( Harv. )Setch.Studies on the extraction of polysaccharides sulfate from three algae and their scavenging activity on free radicalsHAN Hua,ZHOU Hai Yan,LIU Cheng-Chu,WANG Chun-bo(1.Bromatology Institute of Shanhai Aquatic University , Shanghai 200090, China; 2. Medical College , Qingdao University ,Qingdao 266021, China)Abstract:Objective The polysaccharides sulfate were extracted from three kinds of algae in-cluding porphyra tenera , Laminaria ja ponica and Sargassum fusi forme ( Harv. )Setch . in order to screen antioxidant with exploiting potential. Methods Using the assay system of DP-PH,the antioxidative activities of various extracts were studied. Results Three kinds of algae polysaccharides sulfate had different antioxidative activities. Moreover, polysaccharide extracted with different solvents had different intensities of antioxidative activity. Conclusion Polysacchride sulfate extracted with water has stronger antioxidative activity than that with others.